Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells
نویسندگان
چکیده
Cerium dioxide (CeO2) and silicon dioxide (SiO2) nanoparticles are of widespread use in modern life. This means that human beings are markedly exposed to them in their everyday life. Once passing biological barriers, these nanoparticles are expected to interact with endothelial cells, leading to systemic alterations with distinct influences on human health. In the present study we observed the metabolic impact of differently sized CeO2 (8 nm; 35 nm) and SiO2 nanoparticles (117 nm; 315 nm) on immortalized human microvascular (HMEC-1) and primary macrovascular endothelial cells (HUVEC), with particular focus on the CeO2 nanoparticles. The characterization of the CeO2 nanoparticles in cell culture media with varying serum content indicated a steric stabilization of nanoparticles due to interaction with proteins. After cellular uptake, the CeO2 nanoparticles were localized around the nucleus in a ring-shaped manner. The nanoparticles revealed concentration and time, but no size-dependent effects on the cellular adenosine triphosphate levels. HUVEC reacted more sensitively to CeO2 nanoparticle exposure than HMEC-1. This effect was also observed in relation to cytokine release after nanoparticle treatment. The CeO2 nanoparticles exhibited a specific impact on the release of diverse proteins. Namely, a slight trend towards pro-inflammatory effects, a slight pro-thrombotic impact, and an increase of reactive oxygen species after nanoparticle exposure were observed with increasing incubation time. For SiO2 nanoparticles, concentration- and time-dependent effects on the metabolic activity as well as pro-inflammatory reactions were detectable. In general, the effects of the investigated nanoparticles on endothelial cells were rather insignificant, since the alterations on the metabolic cell activity became visible at a nanoparticle concentration that is by far higher than those expected to occur in the in vivo situation (CeO2 nanoparticles: 100 µg/mL; SiO2 nanoparticles: 10 µg/mL).
منابع مشابه
Effect of Nanoparticles on Thin-film Composite Membrane Surface Morphology and Productivity
To evaluate the significance of thin-film composite membrane surface morphology on water productivity, flat-sheet experiments were conducted with silicon dioxide, titanium dioxide, and cerium dioxide nanoparticles. The polyamide thin-film active layer exhibited a valley and ridge morphology that was directly related to the surface roughness, and was found to contribute to particle accumulation ...
متن کاملFate of cerium dioxide nanoparticles in endothelial cells: exocytosis
Although cytotoxicity and endocytosis of nanoparticles have been the subject of numerous studies, investigations regarding exocytosis as an important mechanism to reduce intracellular nanoparticle accumulation are rather rare and there is a distinct lack of knowledge. The current study investigated the behavior of human microvascular endothelial cells to exocytose cerium dioxide (CeO2) nanopart...
متن کاملNovel silicon dioxide -based nanocomposites as an antimicrobial in poly (lactic acid) nanocomposites films
Objective(s): Due to nanocomposites antimicrobial properties, one of the most extensive usages of nano-products is in packing industry. Thus, the production of packages with nanotechnology can effectively prevent against a variety of microorganisms. In this study, the silicon dioxide nanoparticles the poly (lactic acid) PLA films on antimicrobial and permeability was investigated. Methods...
متن کاملPhysico-mechanical and antimicrobial properties of quince seed mucilage supplemented with titanium dioxide and silicon oxide nanoparticles
Objective(s): Quince seed mucilage (QSM) serves as a new source of hydrocolloid which extracted from outer pericarp of Cydonia oblonga seeds upon wetting. It has been traditionally used for the treatment of diseases such as pharyngeal disorder, common cold, colic ulcer, and diarrhea. The aim of the present study was to evaluate the physico-mechanical and antimicrobial properties of quince seed ...
متن کاملPreparation and Characterization of Cerium Doped Titanium Dioxide Nanoparticles by the Electrical Discharge Method
Titanium dioxide nanoparticles (TiO2) are known as a widely used photocatalyst. In order to improve the performance of these nanoparticles, the recombination of the electron-cavity pair must be reduced and the absorption rate of the visible region should be expanded. One way to increase the performance of these nanoparticles is using cerium doped TiO2. In the present study, pure and doped titan...
متن کامل